Rat TH / Tyrosine Hydroxylase

Mouse

Rat, Human, Monkey, Mouse (tested or 100% immunogen sequence identity)

IgM Monoclonal

Unconjugated

Immunoaffinity purified

Unmodified

Rat tyrosine hydroxylase.

C-Terminus

Recognizes an epitope within the catalytically-active C-terminal end-product. This C-terminal product is ~34kD. (This end product is the result of a partial digestion of the rat TH with chymotrypsin.) Species cross-reactivity: Human, primate, mouse.

• IHC
• IHC - Frozen
• Western blot (1:1000 - 1:2000)

Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000-1:2000. Immunohistochemistry: 1:1000 -1:2000 in PBS/0.3% Triton X-100 Cy3 Technique 1:4000-1:8000 in PBS/0.3% Triton X-100 Bn-Av/HRP Technique. Control Tissue: Rat striatum and substantia nigra. Perfusion Fixation: Fixative-4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4. Post Fixation-1.5 hr. at 4? C in 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4. Sections: 10um cryostat frozen sections or 50um vibratome sections.

Lyophilized

100 µl deionized water

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07101 NCBI: NM_000360 NP_000351.2