Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
TGM2 Antibody, G-ALPHA-h Antibody, GNAH Antibody, TG(C) Antibody, Transglutaminase 2 Antibody, Transglutaminase C Antibody, TG2 Antibody, TGase H Antibody, TGase-2 Antibody, TGase-H Antibody, Transglutaminase H Antibody, TGase C Antibody, TGC Antibody, TTG Antibody, C polypeptide Antibody, Tgase II Antibody, Tissue transglutaminase Antibody, Transglutaminase-2 Antibody
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TGM2 immunoreactivity is localized to Cytoplasm significantly.
TGM2 Antibody (Center K444) western blot of HUVEC,K562 cell line lysates (35 ug/lane). The TGM2 antibody detected the TGM2 protein (arrow).