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Anti-TGM2 / Transglutaminase 2 Antibody (Lys444) LS-C97227

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TGM2 / Transglutaminase 2 Antibodies

Anti-TGM2 / Transglutaminase 2 Antibody (clone CUB 7402, Azide-free) LS-C87956
Mouse Monoclonal [clone CUB 7402] (IgG1,k) to Guinea pig TGM2 / Transglutaminase 2
Guinea pig, Human, Dog, Rabbit
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Blocking
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-TGM2 / Transglutaminase 2 Antibody (Azide-free) IHC-plus™ LS-B2681
Rabbit Polyclonal (IgG) to Human TGM2 / Transglutaminase 2
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-TGM2 / Transglutaminase 2 Antibody IHC-plus™ LS-B2235
Rabbit Polyclonal (IgG) to Human TGM2 / Transglutaminase 2
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human TGM2 / Transglutaminase 2
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human TGM2 / Transglutaminase 2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

TGM2 / Transglutaminase 2 antibody was raised against kLH-conjugated synthetic peptide between amino acids 429-458 from internal region of human Transglutaminase (TGM2).
LS-E9294 - Lyophilized - 100 µg - $145.00
Lys444

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About TGM2 / Transglutaminase 2

P21980 NM_004613 NP_004604.2

TGM2 Antibody, G-ALPHA-h Antibody, GNAH Antibody, TG(C) Antibody, Transglutaminase 2 Antibody, Transglutaminase C Antibody, TG2 Antibody, TGase H Antibody, TGase-2 Antibody, TGase-H Antibody, Transglutaminase H Antibody, TGase C Antibody, TGC Antibody, TTG Antibody, C polypeptide Antibody, Tgase II Antibody, Tissue transglutaminase Antibody, Transglutaminase-2 Antibody

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TGM2 immunoreactivity is localized to Cytoplasm significantly.

Western blot

Western blot
TGM2 Antibody (Center K444) western blot of HUVEC,K562 cell line lysates (35 ug/lane). The TGM2 antibody detected the TGM2 protein (arrow).
 

Requested From: United States
Date Requested: 12/7/2016

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