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Anti-TGFB1 / TGF Beta 1 Antibody (clone 2AR2, Biotin) LS-C10855

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $455
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Mouse Monoclonal [clone 2AR2] (IgG1) to Human TGFB1 / TGF Beta 1
Human, Mouse, Rat
IHC, Western blot, Flow Cytometry, ELISA, Neutralization
Biotin Conjugated

Details

Human TGFB1 / TGF Beta 1
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2AR2]
Biotin
Protein G purified

Applications

IHC (8 - 25 µg/ml), Western blot (1 - 2 µg/ml), Flow Cytometry (2.5 µg/10E6 cells), ELISA, Neutralization

Specificity and Use

TGFB1 / TGF Beta 1 antibody was raised against purified, CHO cell-derived, recombinant human transforming growth factor beta 1 (TGF-b1) and latent TGF-b1.
Recognizes human TGF-b1 both in its active form and in its latent form when it is complexed with LAP (latency associated peptide.). Neutralizes the biological activity of TGF-b1 and TGF-b1.2. Species Crossreactivity: mouse and rat. In sandwich ELISA, < 2% crossreactivity with recombinant human TGF-b3 and recombinant amphibian TGF-b5 was observed and no crossreactivity with recombinant porcine or recombinant human TGFb2 is observed.
Suitable for use in capture ELISA, Neutralization, Western Blot, Flow cytometry and Immunohistochemistry. ELISA: 0.1-0.4ug/ml as the detection antibody in sandwich ELISA. Neutralization: ~0.3-1ug/ml will neutralize 50% of the bioactivity in the presence of 0.25ng/ml of recombinant human TGFb1 and 7.5ng/ml recombinant mouse IL-4. Measured by its ability to neutralize TGF-b1 inhibition of IL-4 dependent proliferation in the HT-2 mouse T cell line. Western Blot: 1ug/ml. Reducing conditions are not recommended. Flow Cytometry (Intracellular): 2.5ug/ml labels 1x10e6 cells. Validated using PC-3 human prostate cancer cell line fixed with paraformaldehyde and permeablized with saporin. Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin embedded sections of human prostate cancer tissue. Optimal dilutions to be determined by the researcher.

Packaging

PBS, pH 7.2,
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TGFB1 / TGF Beta 1

P01137 NM_000660 NP_000651.3

TGFB1 Antibody, CED Antibody, DPD1 Antibody, Diaphyseal dysplasia 1 Antibody, LAP Antibody, Latency-associated peptide Antibody, TGF Beta Antibody, TGFbeta Antibody, TGF-beta 1 protein Antibody, TGF-beta-1 Antibody, TGFB Antibody

TGFB1 / TGF Beta 1 is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors.

Requested From: United States
Date Requested: 9/28/2016

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