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Anti-TG / Thyroglobulin Antibody (clone 2H11) IHC-plus™ LS-B428

Note: This antibody replaces LS-C18898

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TG / Thyroglobulin Antibodies

Anti-TG / Thyroglobulin Antibody (clone 2H11) IHC-plus™ LS-B428
Mouse Monoclonal [clone 2H11] (IgG1) to Human TG / Thyroglobulin
Human, Mouse, Rat, Dog
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TG / Thyroglobulin Antibody IHC-plus™ LS-B3173
Mouse Monoclonal (IgG2a) to Human TG / Thyroglobulin
Human, Bovine
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TG / Thyroglobulin Antibody LS-C149169
Rabbit Polyclonal to Human TG / Thyroglobulin
Human
ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2H11] (IgG1) to Human TG / Thyroglobulin
Human, Mouse, Rat, Dog
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated

Details

Human TG / Thyroglobulin
Mouse
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2H11]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen (1:50 - 1:200)
  • Western blot (1:400)
  • ELISA

Specificity and Use

TG / Thyroglobulin antibody was raised against human thyroglobulin protein.
Human thyroglobulin protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with thyroglobulin from human thyroid gland using conventional hybridoma technology.
Immunohistochemistry: LS-B428 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B428 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About TG / Thyroglobulin

P01266 NM_003235 NP_003226.4

TG Antibody, AITD3 Antibody, TDH3 Antibody, Thyroglobulin Antibody, TGN Antibody

Thyroglobulin (TG) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen.

Immunohistochemistry

Immunohistochemistry
Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B428 concentration 5 ug/ml.

Western blot

Western blot
Anti-Thyroglobulin Antibody - Western Blot. Western blot of Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kD band corresponding to human thyroglobulin (left lane 3 ug, right lane 3.0 ug) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.

Western blot

Western blot
Western blot of anti-Thyroglobulin antibody at a dilution of 1:400. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 mg, right lane 3.0 mg) as confirmed by the position of molecular weight markers (not shown).
 

Requested From: United States
Date Requested: 12/3/2016

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