(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot
TFPI / LACI antibody was raised against nS0-derived rhTFPI.
Detects human TFPI in direct ELISAs and Western Blots. Shows no cross-reactivity with rmTFPI or rhTFPI-2.
Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human TFPI. Using a colorimetric detection system, the detection limit for rhTFPI is approximately 1 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human TFPI. The detection limit for rhTFPI is approximately 2 ng/well. Immunoprecipitation: This antibody was used at a concentration of 25 ug/ml to immunoprecipitate rhTFPI from conditioned cell culture medium. The recovered rhTFPI can be detected in Western blots using a polyclonal antibody.
100 µl deionized water
For research use only.
This antibody carries the LSBio 100% Guarantee.