Target
Human TFPI / LACI
Synonyms
TFPI | Anti-convertin | Extrinsic pathway inhibitor | EPI | LACI | TFI | TFPI1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-VKIAYEEIFVKNM, from the C-Terminus of protein sequence according to NP_006278.1.
Specificity
Human TFPI. This antibody is expected to recognize isoform a (NP_006278.1) only.
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 50 kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0 kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx. 38 kDa to 45 kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee