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Anti-TFAP2C / AP2 Gamma Antibody (aa252-301) IHC-plus™ LS-B3506

Note: This antibody replaces LS-C80056

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TFAP2C / AP2 Gamma Antibodies

Anti-TFAP2C / AP2 Gamma Antibody (aa270-281) IHC-plus™ LS-B2757
Goat Polyclonal to Human TFAP2C / AP2 Gamma
Human, Monkey, Rabbit
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TFAP2C / AP2 Gamma Antibody (aa252-301) IHC-plus™ LS-B3506
Rabbit Polyclonal (IgG) to Human TFAP2C / AP2 Gamma
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TFAP2C / AP2 Gamma
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TFAP2C / AP2 Gamma
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Drosophila (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

TFAP2C / AP2 Gamma antibody was raised against synthetic peptide located between aa252-301 of human TFAP2C (Q92754, NP_003213). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Seabass, Salmon, Stickleback, Zebrafish (100%); Panda, Lizard, Drosophila (92%).
LS-E11812 - Lyophilized - 100 µg - $145.00
aa252-301
Human TFAP2C / AP2 Gamma
Immunohistochemistry: LS-B3506 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3506 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TFAP2C / AP2 Gamma

Q92754 NM_003222 NP_003213.1

TFAP2C Antibody, AP2 Gamma Antibody, AP-2 gamma Antibody, ERF-1 Antibody, ERF1 Antibody, HAP-2g Antibody, Transcription factor ERF-1 Antibody, TFAP2G Antibody, AP2-GAMMA Antibody, Estrogen receptor factor 1 Antibody

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.

Immunohistochemistry

Immunohistochemistry
Anti-TFAP2C / AP2 Gamma antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3506 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-TFAP2C / AP2 Gamma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3506 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-TFAP2C / AP2 Gamma antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3506 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/7/2016

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