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Anti-TF / Transferrin Antibody IHC-plus™ LS-B2447

Note: This antibody replaces LS-C39807

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TF / Transferrin
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human TF / Transferrin
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Ion exchange chromatography

Applications

IHC - Paraffin (5 µg/ml), Western blot, Immunoprecipitation, ELISA (1:40000 - 1:160000)

Specificity and Use

TF / Transferrin antibody was raised against transferrin (Human Serum).
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum, purified and partially purified transferrin (human serum). Cross-reactivity against transferrin from other tissues and species may occur by have not been tested.
Immunohistochemistry: LS-B2447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2447 was determined to be 5 ug/ml. ELISA: 1:40000-1:160000 (Coat with 1 ug transferrin in a standard sandwich ELISA using peroxidase conjugated rabbit IgG and ABTS as a substrate for 30 min at RT).

Packaging

PBS, pH 7.2, 0.01% sodium azide, 40% glycerol.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About TF / Transferrin

P02787 NM_001063 NP_001054.1

TF Antibody, PRO1557 Antibody, Serotransferrin Antibody, Siderophilin Antibody, TFQTL1 Antibody, Beta-1 metal-binding globulin Antibody, Transferrin Antibody, PRO2086 Antibody

TF / Transferrin is a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body.

Immunohistochemistry

Immunohistochemistry
Anti-TF / Transferrin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2447 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 7/31/2015

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