Target
Pig TF / Transferrin
Synonyms
TF | PRO1557 | Serotransferrin | Siderophilin | TFQTL1 | Beta-1 metal-binding globulin | Transferrin | PRO2086
Reactivity
Pig
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase.
Also available
Unconjugated or conjugated with
FITC,
Biotin.
Purification
Purified IgG
Immunogen
Purified transferrin isolated from pooled swine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Swine transferrin.
Applications
- IHC
- IHC - Paraffin
- ICC
- ELISA
- Dot Blot
- Immunodiffusion
- Applications tested for the base form of this product only
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Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is restricted to transferrin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin. In enzyme-immunocytochemical and immunohistochemical techniques for the detection of transferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates. as detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:5000.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee