Human TF / Transferrin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Immunoaffinity purified

Unmodified

Purified, Sfr-derived, recombinant human Transferrin (rhTransferrin; aa 20-698; Accession# P02787).

aa20-698

Detects rhTransferrin in direct ELISAs and Western blots.

• Western blot (2 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in ELISA, Suitable for use in Western blot: 2 ug/ml with the appropriate secondary reagents to detect human Transferrin in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from human liver extract in non-reducing sample buffer, resolved by SDS-PAGE (30 ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with 2 ug/ml and. chemiluminescent detection substrate. Use of this antibody under reducing condition is not. recommended. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human Transferrin. The detection limit for rhTransferrin is approximately 1 ng/well.

Lyophilized from Sterile Solution PBS, 5% Trehalose

Reconstitute in sterile PBS.

Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02787 NCBI: NM_001063 NP_001054.1