Target
Human TF / Transferrin
Synonyms
TF | PRO1557 | Serotransferrin | Siderophilin | TFQTL1 | Beta-1 metal-binding globulin | Transferrin | PRO2086
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Purified, Sfr-derived, recombinant human Transferrin (rhTransferrin; aa 20-698; Accession# P02787).
Specificity
Detects rhTransferrin in direct ELISAs and Western blots.
Applications
- Western blot (2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in ELISA, Suitable for use in Western blot: 2 ug/ml with the appropriate secondary reagents to detect human Transferrin in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from human liver extract in non-reducing sample buffer, resolved by SDS-PAGE (30 ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with 2 ug/ml and. chemiluminescent detection substrate. Use of this antibody under reducing condition is not. recommended. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human Transferrin. The detection limit for rhTransferrin is approximately 1 ng/well.
Presentation
Lyophilized from Sterile Solution PBS, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee