Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Western blot (1 - 10 µg/ml)
ELISA (0.5 - 1 µg/ml)
A 21 amino acid peptide sequence mapping in the middle of human Est2 (KLH coupled). No significant sequence homology with mouse Est2.
Recognizes human Telomerase.
Suitable for use in ELISA and Western Blot. Western Blot: 1-10 ug/ml using ECL. The antibody has detected ~120-130kD band from several tumor cell lines. An antibody made to a region near to the hESt21 epitope recognized ~125kD band in human and mouse tissues. ELISA: 0.5-1 ug/ml. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.