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Anti-TEK / TIE2 Antibody (phospho-Tyr992) LS-C152798

Ordering

Wt. Vol. Conc. Price
100 µg - - $345
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TEK / TIE2 Antibodies

Anti-TEK / TIE2 Antibody (clone TEK4) LS-C107063
Rat Monoclonal [clone TEK4] (IgG1,k) to Mouse TEK / TIE2
Mouse
IHC - Frozen, Flow Cytometry
Unconjugated
Anti-TEK / TIE2 Antibody (aa23-745) LS-C150086
Goat Polyclonal (IgG) to Human TEK / TIE2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
IHC, Western blot, Immunoprecipitation
Unconjugated

Details

Human TEK / TIE2
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • Immunoprecipitation

Specificity and Use

TEK / TIE2 antibody was raised against synthetic peptide encompassing and including phosphorylated Tyr992 of human TIE2
pTyr992
Recognizes TIE2/TEK phosphorylated on Tyr992

Packaging

PBS, 0.05% sodium azide in 50% glycerol
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.
For research use only.

About TEK / TIE2

Q02763 NM_000459 NP_000450.2

TEK Antibody, Angiopoietin-1 receptor Antibody, CD202b antigen Antibody, CD202B Antibody, Endothelial tyrosine kinase Antibody, HTIE2 Antibody, Hyk Antibody, Soluble TIE2 variant 1 Antibody, Soluble TIE2 variant 2 Antibody, TIE-2 Antibody, TIE2 Antibody, VMCM Antibody, Tek tyrosine kinase Antibody, VMCM1 Antibody, p140 TEK Antibody

Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis.

Western blot

Western blot
WB: Untreated and pervanadate-treated HUVEC (lanes 1 and 2, respectively) were resolved by SDS-PAGE, transferred to PVDF (Immobilon-P), and probed with anti-phospho-TIE2 (Tyr992) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated HUVEC lysate was either unblocked, TIE2 Tyr992 phosphorylated-peptide blocked, or TIE2 non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-TIE2 (Tyr992) (1:1,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated TIE2.
 

Requested From: United States
Date Requested: 12/7/2016

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