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Anti-TEK / TIE2 Antibody (aa23-745) LS-C150086

Ordering

Wt. Vol. Conc. Price
100 µg - 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular TEK / TIE2 Antibodies

Anti-TEK / TIE2 Antibody (clone TEK4) LS-C107063
Rat Monoclonal [clone TEK4] (IgG1,k) to Mouse TEK / TIE2
Mouse
IHC - Frozen, Flow Cytometry
Unconjugated
Anti-TEK / TIE2 Antibody (aa23-745) LS-C150086
Goat Polyclonal (IgG) to Human TEK / TIE2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human TEK / TIE2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human TEK / TIE2
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • ELISA (10 µg/ml)

Specificity and Use

TEK / TIE2 antibody was raised against mouse myeloma cell line NS0-derived recombinant human Tie-2 Ala23-Lys745 Accession # Q02763.
aa23-745
Detects human Tie2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with.
Western Blot, 0.1 µg/ml, Recombinant Human Tie-2 Fc Chimera. Immunohistochemistry, 5-15 µg/ml, Blockade of Receptor-ligand Interaction, In a functional ELISA, 5-10 µg/ml of this antibody will block 50% of the binding of 40 ng/ml of biotinylated. Recombinant Human Angiopoietin-2 to immobilized Recombinant Human Tie-2 Fc Chimera coated at 4 µg/ml (100 ul/well). At 50 µg/ml, this antibody will block >90% of the binding.

Packaging

Lyophilized from 0.2 um sterile-filtered solution in PBS, pH 7.4, 5% trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TEK / TIE2

Q02763 NM_000459 NP_000450.2

TEK Antibody, Angiopoietin-1 receptor Antibody, CD202b antigen Antibody, CD202B Antibody, Endothelial tyrosine kinase Antibody, HTIE2 Antibody, Hyk Antibody, Soluble TIE2 variant 1 Antibody, Soluble TIE2 variant 2 Antibody, TIE-2 Antibody, TIE2 Antibody, VMCM Antibody, Tek tyrosine kinase Antibody, VMCM1 Antibody, p140 TEK Antibody

Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis.

Immunohistochemistry

Immunohistochemistry
Tie-2 in Human Placenta. Tie-2 was detected in immersion fixed paraffin-embedded sections of human placenta using Human Tie-2 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4 °C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).
 

Requested From: United States
Date Requested: 12/8/2016

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