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Anti-TEAD4 Antibody (C-Terminus) IHC-plus™ LS-B3522

Note: This antibody replaces LS-C30220
Catalog Size Price
LS-B3522-50 50 µg (1 mg/ml) Unavailable

Related Products

22 TEAD4 Antibodies

Most Popular TEAD4 Antibodies

Anti-TEAD4 Antibody (C-Terminus) IHC-plus™ LS-B3522
Rabbit Polyclonal (IgG) to Human TEAD4
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TEAD4 Antibody (clone 1D10) LS-C133476
Mouse Monoclonal [clone 1D10] (IgG1,k) to Human TEAD4
Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-TEAD4 Antibody (aa151-261, Azide-free) LS-C328857
Mouse Monoclonal (IgG2a,k) to Human TEAD4
Human
Western blot, ELISA
Unconjugated, Azide-free
Anti-TEAD4 Antibody (aa74-434) LS-C371369
Rabbit Polyclonal (IgG) to Human TEAD4
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TEAD4 Antibody (aa74-434, HRP) LS-C371371
Rabbit Polyclonal (IgG) to Human TEAD4
Human
IHC - Paraffin, Western blot, ELISA
HRP Conjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal (IgG) to Human TEAD4
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TEAD4
Rabbit
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Monkey, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TEAD4 antibody was raised against synthetic peptide from C-Terminus of human TEAD4 (Q96BK2, NP_003204). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%); Marmoset, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (92%); Lizard (85%).
LS-E13749 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3522. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
Human TEAD4
Immunohistochemistry: LS-B3522 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3522 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TEAD4

Q15561 NM_003213 NP_003204.2

TEAD4 Antibody, EFTR-2 Antibody, HRTEF-1B Antibody, RTEF1 Antibody, RTEF-1 Antibody, TCF13L1 Antibody, TEF-3 Antibody, TEAD-4 Antibody, TEF3 Antibody, Transcription factor 13-like 1 Antibody, Transcription factor RTEF-1 Antibody, TEFR-1 Antibody, TEA domain family member 4 Antibody

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ.

Immunohistochemistry

Immunohistochemistry
Anti-TEAD4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3522 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-TEAD4 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3522 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
293T Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 8/20/2017

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