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Anti-TCR Vbeta 16.1 Antibody (clone HIS42) LS-C58504

Note: This antibody replaces LS-C76314

Ordering

Wt. Vol. Conc. Price
- 2000 µl - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular TCR Vbeta 16.1 Antibodies

Anti-TCR Vbeta 16.1 Antibody (clone HIS42) LS-C58504
Mouse Monoclonal [clone HIS42] (IgG2b) to Rat TCR Vbeta 16.1
Rat
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone HIS42] (IgG2b) to Rat TCR Vbeta 16.1
Rat
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Rat TCR Vbeta 16.1
Mouse
Rat (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [HIS42]
Unconjugated
Unmodified

Applications

  • IHC - Frozen (1:10)
  • Immunoprecipitation
  • Flow Cytometry

Specificity and Use

TCR Vbeta 16.1 antibody was raised against pVG RT7.2 Rat Thymocytes.
Detects a minor subset of T cells. The subpopulation of HIS 42 positive T cells contains both CD4 and CD8 positive cells, in the same ratio as found in the peripheral T cell population. When bound to Sepharose beads, HIS 42 induces T cell proliferation in the presence of IL-2. In contrast to lymph node T cells a number of thymocytes are found to express HIS 42 only in the cytoplasm or together with membrane expression. Most bright HIS 42 surface labeled thymocytes are also positive for MRC OX-44, a marker predominantly identifying mature thymocytes. SDS PAGE analysis of the membrane molecules immunoprecipitated by HIS 42 shows a band at 85kD. On reduction it separates to two bands at 35kD and 48kD.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.

Packaging

Tissue culture supernatant, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About TCR Vbeta 16.1


Requested From: United States
Date Requested: 12/8/2016

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