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Anti-TCR Valpha 2 Antibody (clone B20.1) LS-C106630

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable
100 µg - - Unavailable
500 µg - - Unavailable

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100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone B20.1] (IgG2a) to Mouse TCR Valpha 2
Mouse
Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Mouse TCR Valpha 2
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [B20.1]
Unconjugated
Affinity purified
Unmodified

Applications

  • Immunoprecipitation
  • Flow Cytometry

Specificity and Use

TCR Valpha 2 antibody was raised against mouse T Cell Receptor Valpha 2
This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to preblock Fc receptors for 10 minutes using 0.5 ug of purified anti-mouse CD16/32 (LS-C107169). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging

PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.

About TCR Valpha 2


Flow Cytometry

Flow Cytometry
Staining of C57Bl/6 lymph node cells with 0.125 ug of Purified Rat IgG2a isotype control (open histogram) or 0.125 ug of Purified anti-mouse Va-2 TCR (B20.1) (colored histogram) followed by PE Anti-Rat IgG (LS-C106534). Total viable cells were used for analysis.
 

Requested From: 
Date Requested: 3/28/2017

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