TCP1 antibody LS-C18046 is an unconjugated rat monoclonal antibody to TCP1 from human, mouse, rat and other species. Validated for ICC, IP and WB.
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Drosophila
(tested or 100% immunogen sequence identity)
Protein G purified
- ICC (5 µg/ml)
- Western blot
- Immunoprecipitation (12.5 µg/ml)
TCP1 antibody was raised against a purified recombinant mouse TCP-1alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp-1b gene expressed in E. coli.
This affinity purified antibody recognizes an epitope in the C-terminus of mouse TCP-1a (465-AKLRA) and will detect an ~60kD protein, corresponding to the apparent molecular mass of TCP-1alpha on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, porcine and rabbit origins, as well as Saccharomyces cerevisiae (weakly, consistent with the epitope sequence being AKLRS), Drosophila, Schizosaccharomyces pombe and C. elegans. In C. elegans CTA-191 reacts with TCP-1a and another CCT subunit. In plants, CTA-191 recognizes TCP-1 of Pisum sativum and the sequence of Arabidopsis thalania TCP-1a over the region of the epitope AKLRA. It has also been shown that CTA-191 reacts with a subunit of a specialized chaperonin which folds phytochrome. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis.
Western Blot (Colorimetric): 1 ug/ml. Immunoprecipitation: 12.5 ug/ml 5 ug/ml. Immunocytochemistry: 5 ug/ml. Positive control: HeLa Heat Shocked Cell Lysate.
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.