Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Drosophila
(tested or 100% immunogen sequence identity)
Protein G purified
ICC (5 µg/ml)
Immunoprecipitation (12.5 µg/ml)
TCP1 antibody was raised against a purified recombinant mouse TCP-1alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp-1b gene expressed in E. coli.
This affinity purified antibody recognizes an epitope in the C-terminus of mouse TCP-1a (465-AKLRA) and will detect an ~60kD protein, corresponding to the apparent molecular mass of TCP-1alpha on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, porcine and rabbit origins, as well as Saccharomyces cerevisiae (weakly, consistent with the epitope sequence being AKLRS), Drosophila, Schizosaccharomyces pombe and C. elegans. In C. elegans CTA-191 reacts with TCP-1a and another CCT subunit. In plants, CTA-191 recognizes TCP-1 of Pisum sativum and the sequence of Arabidopsis thalania TCP-1a over the region of the epitope AKLRA. It has also been shown that CTA-191 reacts with a subunit of a specialized chaperonin which folds phytochrome. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis.
Western Blot (Colorimetric): 1 ug/ml. Immunoprecipitation: 12.5 ug/ml 5 ug/ml. Immunocytochemistry: 5 ug/ml. Positive control: HeLa Heat Shocked Cell Lysate.
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
TCP1 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.