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Anti-TCF4 Antibody (aa73-122) IHC-plus™ LS-B1570

Note: This antibody replaces LS-C30471

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TCF4 Antibodies

Anti-TCF4 Antibody (aa73-122) IHC-plus™ LS-B1570
Rabbit Polyclonal (IgG) to Human TCF4
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TCF4 Antibody (N-Terminus) IHC-plus™ LS-B8267
Rabbit Polyclonal (IgG) to Human TCF4
Human, Monkey, Mouse, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TCF4 Antibody (aa55-84, APC) LS-C271204
Rabbit Polyclonal (IgG) to Human TCF4
Human
Western blot, Flow Cytometry, ELISA
APC Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal (IgG) to Human TCF4
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TCF4
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Guinea pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TCF4 antibody was raised against synthetic peptide located between aa73-122 of human TCF4 (P15884, NP_003190). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum (100%); Guinea pig (92%).
LS-E10834 - Lyophilized - 100 µg - $145.00
aa73-122
Human TCF4
Immunohistochemistry: LS-B1570 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1570 was determined to be 5 ug/ml.

Packaging

Lyophilized from 2% Sucrose, PBS.
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
Primary fibroblasts of NDUFS4(-/-) mice display increased ROS levels and aberrant mitochondrial morphology. Valsecchi F, Grefte S, Roestenberg P, Joosten-Wagenaars J, Smeitink JA, Willems PH, Koopman WJ. Mitochondrion. 2013 Sep;13:436-43. [Full Text Article] [PubMed:23234723]

About TCF4

P15884 NM_003199 NP_003190.1

TCF4 Antibody, BHLHb19 Antibody, E2-2 Antibody, ITF-2 Antibody, ITF2 Antibody, PTHS Antibody, SEF2-1B Antibody, SEF2-1 Antibody, SEF2-1A Antibody, SL3-3 enhancer factor 2 Antibody, SEF2 Antibody, SEF-2 Antibody, TCF-4 Antibody, Transcription factor 4 Antibody

TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box (&#039E-box&#039) binding site (&#039CANNTG&#039) - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well.

Immunohistochemistry

Immunohistochemistry
Anti-TCF4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1570 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
TCF4 antibody LS-B1570 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/4/2016

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