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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TCF12 / HEB Antibody (N‑Terminus) IHC‑plus™ LS‑B5344
Note: This antibody replaces LS-C115915
HEB antibody LS-B5344 is an unconjugated rabbit polyclonal antibody to HEB (TCF12) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5344-50
50 µg (1 mg/ml)
Unavailable

Popular TCF12 / HEB Products

TCF12 antibody LS-C112350 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Applications: Western blot
Immunoperoxidase of monoclonal antibody to TCF12 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-TCF12 / HEB antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10267 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Detection of Human TCF12 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TCF12 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

HEB antibody LS-B5344 is an unconjugated rabbit polyclonal antibody to HEB (TCF12) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About TCF12 / HEB
TCF12 / HEB is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Q99081 NM_003205 NP_003196.1

TCF12 Antibody, BHLHb20 Antibody, E-box-binding protein Antibody, HEB Antibody, HsT17266 Antibody, HTF4 Antibody, TCF-12 Antibody, Transcription factor 12 Antibody, Transcription factor HTF-4 Antibody, DNA-binding protein HTF4 Antibody


Specifications

Target
Human TCF12 / HEB
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E31011 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B5344. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Usage
TCF12 antibody can be used for detection of TCF12 by Western blot at 0.5-1 ug/ml.
Presentation
PBS, 0.02% sodium azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-TCF12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5344 concentration 5 ug/ml.
Anti-TCF12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5344 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 µg/mL.
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 ug/ml.
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 ug/ml.

Western blot

Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 µg/mL.
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 µg/mL.

Western blot

Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunohistochemistry

Anti-TCF12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5344 concentration 5 ug/ml.
Anti-TCF12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5344 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 µg/mL.
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 ug/ml.
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 ug/ml.

Western blot

Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 µg/mL.
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 µg/mL.

Western blot

Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunohistochemistry

Anti-TCF12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5344 concentration 5 ug/ml.
Anti-TCF12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5344 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 µg/mL.
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 ug/ml.
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 ug/ml.

Western blot

Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 µg/mL.
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 µg/mL.

Western blot

Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunohistochemistry

Anti-TCF12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5344 concentration 5 ug/ml.
Anti-TCF12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5344 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 µg/mL.
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 ug/ml.
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 ug/ml.

Western blot

Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 µg/mL.
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 µg/mL.

Western blot

Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 ug/ml.


Requested From: 
Date Requested: 6/19/2018

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