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Anti-TBX21 / T-bet Antibody (N-Terminus) LS-C116073


Wt. Vol. Conc. Price
50 µg - - Unavailable

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Rabbit Polyclonal to Mouse TBX21 / T-bet
Mouse, Human, Bovine, Dog, Pig, Rabbit
Western blot (applications tested for the base form of this product only)


Mouse TBX21 / T-bet
Mouse, Human, Bovine, Dog, Pig, Rabbit (tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Immunoaffinity purified


  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

  • IHC - Paraffin

Specificity and Use

TBX21 / T-bet antibody was raised against synthetic peptide from N-Terminus of mouse Tbx21 (Q9JKD8, NP_062380). Percent identity by BLAST analysis: Mouse (100%); Rat (92%); Human, Gorilla, Marmoset, Rabbit (85%); Galago (84%).
LS-E13355 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116073. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Mouse TBX21 / T-bet
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.


Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TBX21 / T-bet

Q9UL17 NM_013351 NP_037483.1

TBX21 Antibody, T-bet Antibody, TBET Antibody, T-box 21 Antibody, T-box protein 21 Antibody, TBLYM Antibody, Transcription factor TBLYM Antibody, T-box expressed in T cells Antibody, T-PET Antibody

TBX21 / T-bet is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG).

Western blot

Western blot
TBX21 / T-bet antibody LS-C116073 Western blot of NIH/3T3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/23/2017

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