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Anti-TBP / GTF2D Antibody (aa39-50) LS-C154977

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TBP / GTF2D Antibodies

Anti-TBP / GTF2D Antibody LS-C39506
Rabbit Polyclonal to Human TBP / GTF2D
Human
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human TBP / GTF2D
Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Western blot, ELISA
Unconjugated

Details

Human TBP / GTF2D
Goat
Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Rat, Guinea pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 - 3 µg/ml)
  • ELISA (1:8000)

Specificity and Use

TBP / GTF2D antibody was raised against synthetic peptide C-TPQPIQNTNSLS from an internal region of human TBP (NP_003185.1; NP_001165556.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Porcine (100%); Rat, Elephant, Guinea pig, Stickleback, Medaka, Pufferfish (92%).
LS-E27847 - Lyophilized - 100 µg - $145.00
aa39-50
Human TBP. This antibody is expected to recognize both reported isoforms (NP_003185.1; NP_001165556.1).
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 40kD band observed in nuclear, but not in cytosolic lysates of cell line HeLa (calculated MW of 37.7kD according to NP_003185.1). Recommended concentration: 1-3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About TBP / GTF2D

P20226 NM_003194 NP_003185.1

TBP Antibody, GTF2D1 Antibody, HDL4 Antibody, TATA box binding protein Antibody, TATA-binding factor Antibody, TATA-box factor Antibody, TFIID Antibody, TATA sequence-binding protein Antibody, SCA17 Antibody, TATA-box-binding protein Antibody, GTF2D Antibody, TF2D Antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals.

Western blot

Western blot
TBP antibody (1 ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/9/2016

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