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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TAGLN / Transgelin / SM22 Antibody (aa139‑167) LS‑C98414
SM22 antibody LS-C98414 is an unconjugated rabbit polyclonal antibody to SM22 (TAGLN / Transgelin) from human and mouse. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C98414-400
400 µl
Unavailable

Popular TAGLN / Transgelin / SM22 Products

Anti-TAGLN / SM22 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2563 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Xenopus
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-TAGLN / SM22 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6880 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat, Human, Monkey, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin
Anti-TAGLN / Transgelin / SM22 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

SM22 antibody LS-C98414 is an unconjugated rabbit polyclonal antibody to SM22 (TAGLN / Transgelin) from human and mouse. Validated for Flow, IHC and WB.
About TAGLN / Transgelin / SM22
TAGLN / Transgelin / SM22 is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. Q01995 NM_003186 NP_003177.2

TAGLN Antibody, 22 kDa actin-binding protein Antibody, SMCC Antibody, TAGLN1 Antibody, WS3-10 Antibody, Transgelin Antibody, Transgelin variant 2 Antibody, SM22 Antibody, Protein WS3-10 Antibody, SM22-alpha Antibody


Specifications

Target
Human TAGLN / Transgelin / SM22
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8377 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C98414. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa139-167
Specificity
This TAGLN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-167 amino acids from the C-terminal region of human TAGLN.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of TAGLN antibody in mouse bladder tissue lysates (35 ug/lane). TAGLN (arrow) was detected using the purified antibody.
Western blot of TAGLN antibody in mouse bladder tissue lysates (35 ug/lane). TAGLN (arrow) was detected using the purified antibody.

Flow Cytometry

TAGLN Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TAGLN Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of TAGLN antibody in mouse bladder tissue lysates (35 ug/lane). TAGLN (arrow) was detected using the purified antibody.
Western blot of TAGLN antibody in mouse bladder tissue lysates (35 ug/lane). TAGLN (arrow) was detected using the purified antibody.

Flow Cytometry

TAGLN Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TAGLN Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of TAGLN antibody in mouse bladder tissue lysates (35 ug/lane). TAGLN (arrow) was detected using the purified antibody.
Western blot of TAGLN antibody in mouse bladder tissue lysates (35 ug/lane). TAGLN (arrow) was detected using the purified antibody.

Flow Cytometry

TAGLN Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TAGLN Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of TAGLN antibody in mouse bladder tissue lysates (35 ug/lane). TAGLN (arrow) was detected using the purified antibody.
Western blot of TAGLN antibody in mouse bladder tissue lysates (35 ug/lane). TAGLN (arrow) was detected using the purified antibody.

Flow Cytometry

TAGLN Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TAGLN Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/23/2018

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