(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Paraffin (0.2 - 0.4 µg/ml)
- Western blot (1 - 2 µg/ml)
- Flow Cytometry
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TAG-72 antibody was raised against membrane-enriched fraction of a human breast carcinoma liver metastasis and purified TAG-72 protein. MW: >1000kD.
Recognizes human TAG-72. Species cross-reactivity: bovine, canine and rat.
Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation, Radioimaging and Immunohistochemistry. Western Blot: 1-2 ug/ml for 2hrs at RT. Immunoprecipitation: Use Protein G, 2 ug/mg protein lysate. Immunohistochemistry, Formalin-fixed, paraffin-embedded: 0.2-0.4 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Ls174T cells. Colon Carcinoma. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2. No stabilizing proteins added. No preservative added.
For research use only.
This antibody carries the LSBio 100% Guarantee.