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Anti-TAF3 Antibody (aa540-589) LS-C102385

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Wt. Vol. Conc. Price
50 µg - - Unavailable

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Anti-TAF3 Antibody (aa540-589) LS-C102385
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Western blot Image
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Rabbit Polyclonal (IgG) to Human TAF3
Human, Monkey, Dog
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TAF3
Rabbit
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
Bat, Guinea pig, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TAF3 antibody was raised against synthetic peptide located between aa540-589 of human TAF3 (Q5VWG9, NP_114129). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%); Gorilla, Beetle (92%); Bat, Rabbit, Guinea pig (91%); Drosophila (86%); Elephant (85%); Zebrafish (84%); Xenopus (80%).
LS-E18449 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102385. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa540-589
Human TAF3
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TAF3

AA777658

TAF3 Antibody, TAFII-140 Antibody, TBP-associated factor 3 Antibody, TAF140 Antibody, TAF(II)140 Antibody, TAFII140 Antibody

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

Western blot

Western blot
TAF3 antibody LS-C102385 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/24/2017

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