Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 323/A3] (IgG1) to Human TACSTD1 / EPCAM
ICC, Western blot, Immunoprecipitation, Flow Cytometry
Human TACSTD1 / EPCAM
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [323/A3]
Protein A purified
IHC - Paraffin
Specificity and Use
TACSTD1 / EPCAM antibody was raised against human breast cancer MCF-7 cells.
The antibody 323/A3 recognizes CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.
Immunoprecipitation: Recommended dilution - 1-4 ug / ml per 100-500 ug of protein in 1 ml lysate. Western Blotting: Recommended dilution - 1-4 ug/ml. Positive control - A-431, MCF-7, SW480 cell lines. Immunohistochemistry (paraffin sections): Recommended
PBS, 15 mM sodium azide, pH 7.4
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.