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Anti-SYVN1 / HRD1 Antibody (aa586-617) IHC-plus™ LS-B3451

Note: This antibody replaces LS-C99633

Ordering

Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $395
Inquire for larger quantities

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Most Popular SYVN1 / HRD1 Antibodies

Anti-SYVN1 / HRD1 Antibody (aa586-617) IHC-plus™ LS-B3451
Rabbit Polyclonal to Human SYVN1 / HRD1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal to Human SYVN1 / HRD1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human SYVN1 / HRD1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)

Specificity and Use

SYVN1 / HRD1 antibody was raised against kLH-conjugated synthetic peptide between amino acids 586-617 from C-terminal region of human SYVN1 (HRD1).
LS-E6927 - Lyophilized - 100 µg - $145.00
aa586-617
Immunohistochemistry: LS-B3451 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3451 was determined to be 10 ug/ml.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (2)

1
Loss of HRD1-mediated protein degradation causes amyloid precursor protein accumulation and amyloid-beta generation. Kaneko M, Koike H, Saito R, Kitamura Y, Okuma Y, Nomura Y. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2010 30:3924-32. [PubMed:20237263]
2
Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels. Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR. The Journal of biological chemistry. 2011 286:41530-8. [PubMed:21987572] [PMC:PMC3308864]

About SYVN1 / HRD1

Q86TM6 NM_032431 NP_115807.1

SYVN1 Antibody, KIAA1810 Antibody, Synoviolin Antibody, Synovial apoptosis inhibitor 1 Antibody, DER3 Antibody, HRD1 Antibody

Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK.

Immunohistochemistry

Immunohistochemistry
Anti-SYVN1 / HRD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3451 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-SYVN1 / HRD1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3451 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SYVN1 (HRD1) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).

Western blot

Western blot
The anti-HRD1 antibody is used in Western blot to detect HRD1 in mouse kidney tissue lysate.

Western blot

Western blot
Western blot of SYVN1 (HRD1) Antibody in T47D cell line lysates (35 ug/lane). HRD1 (arrow) was detected using the purified antibody.

Western blot

Western blot
Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. Primary antibodies against syvn1 (1:1000) and anti-rabbit secondary POD-conjugated antibodies from Pierce Biotechnology, Inc (Rockford, IL, 1:2000)(Provided by Dr. Susana Granell & Institution University of Arkansas).

Requested From: United States
Date Requested: 12/9/2016

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