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Catalog Number	Size	Price
LS-B3451-200	200 µl (1 mg/ml)	$460

SYVN1 / HRD1 Antibody - Anti-SYVN1 / HRD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

SYVN1 / HRD1 Antibody - Anti-SYVN1 / HRD1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

SYVN1 / HRD1 Antibody - Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SYVN1 (HRD1) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).

SYVN1 / HRD1 Antibody - The anti-HRD1 antibody is used in Western blot to detect HRD1 in mouse kidney tissue lysate.

SYVN1 / HRD1 Antibody - Western blot of SYVN1 (HRD1) Antibody in T47D cell line lysates (35 ug/lane). HRD1 (arrow) was detected using the purified antibody.

SYVN1 / HRD1 Antibody - Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. Primary antibodies against syvn1 (1:1000) and anti-rabbit secondary POD-conjugated antibodies from Pierce Biotechnology, Inc (Rockford, IL, 1:2000)(Provided by Dr. Susana Granell & Institution University of Arkansas).

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IHC‑plus™ Polyclonal Rabbit anti‑Human SYVN1 / HRD1 Antibody (aa586‑617, IHC, IF, WB) LS‑B3451

Name: IHC-plus™ SYVN1 / HRD1 Polyclonal Antibody Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B3451-200
Price: 460.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

IHC Validated

(5.0) Rating

Submit Review (2) Publication(s) LSBio Guarantee

Note: This antibody replaces LS-C99633

Antibody:

SYVN1 / HRD1 Rabbit anti-Human Polyclonal (aa586-617) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$460

LS-B3451-200

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SYVN1 / HRD1 Rabbit anti-Human Polyclonal (aa586-617) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

HRD1 antibody LS-B3451 is an unconjugated rabbit polyclonal antibody to HRD1 (SYVN1) (aa586-617) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.

(2) Publication(s)

Target

Human SYVN1 / HRD1

Synonyms

See All SYVN1 / HRD1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Ammonium sulfate precipitation

Modifications

Unmodified

Epitope

aa586-617

Specificity

This SYVN1 (HRD1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-617 amino acids from the C-terminal region of human SYVN1 (HRD1).

Applications

IHC
IHC - Paraffin (10 µg/ml)
Immunofluorescence (1:200)
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B3451 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3451 was determined to be 10 ug/ml.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About SYVN1 / HRD1

Uniprot: Q86TM6 NCBI: NM_032431 NP_115807.1

Validation

Anti-SYVN1 / HRD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Anti-SYVN1 / HRD1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

See More About...

About LSBio Validation process

LSBio Ratings

IHC-plus™ SYVN1 / HRD1 Antibody (aa586-617) for IHC, IF/Immunofluorescence, WB/Western LS-B3451 has an LSBio Rating of

(5.0) Rating

Publications (4.1)

Laboratory Validation Score (4)

Learn more about The LSBio Ratings Algorithm

Publications (2)

Loss of HRD1-mediated protein degradation causes amyloid precursor protein accumulation and amyloid-beta generation. Kaneko M, Koike H, Saito R, Kitamura Y, Okuma Y, Nomura Y. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2010 30:3924-32.

PubMed: 20237263

Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels. Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR. The Journal of biological chemistry. 2011 286:41530-8.

PubMed: 21987572 PMC: PMC3308864

Submit a Publication for LS-B3451