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Catalog Number Size Price
LS-C357601-10 10 µg $318 
LS-C357601-100 100 µg (0.5 mg/ml) $470 
SYP / Synaptophysin Antibody - Western blot analysis of Synaptophysin using anti-Synaptophysin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptophysin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Synaptophysin at approximately 38KD. The expected band size for Synaptophysin is at 34KD.
SYP / Synaptophysin Antibody - Synaptophysin antibody Western blot. All lanes: Anti Synaptophysin at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Predicted band size: 34 kD. Observed band size: 34 kD.
SYP / Synaptophysin Antibody - Western blot analysis of Synaptophysin using anti-Synaptophysin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptophysin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Synaptophysin at approximately 38KD. The expected band size for Synaptophysin is at 34KD.
SYP / Synaptophysin Antibody - Synaptophysin antibody Western blot. All lanes: Anti Synaptophysin at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Predicted band size: 34 kD. Observed band size: 34 kD.
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Polyclonal Rabbit anti‑Human SYP / Synaptophysin Antibody (aa1‑26, WB) LS‑C357601

Polyclonal Rabbit anti‑Human SYP / Synaptophysin Antibody (aa1‑26, WB) LS‑C357601

Antibody:
SYP / Synaptophysin Rabbit anti-Human Polyclonal (aa1-26) Antibody
Application:
WB
Reactivity:
Human, Orangutan, Gibbon, Monkey, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357601-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SYP / Synaptophysin Rabbit anti-Human Polyclonal (aa1-26) Antibody
Application:
WB
Reactivity:
Human, Orangutan, Gibbon, Monkey, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Synaptophysin antibody LS-C357601 is an unconjugated rabbit polyclonal antibody to Synaptophysin (SYP) (aa1-26) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
Target
Human SYP / Synaptophysin
Synonyms
SYP | MRX | Synaptophysin | MRXSYP
Host
Rabbit
Reactivity
Human, Orangutan, Gibbon, Monkey, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human Synaptophysin(1-26 aa MLLLADMDVVNQLVAGGQFRVVKEPL), identical to the related mouse and rat sequences.
Epitope
aa1-26
Specificity
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SYP / Synaptophysin
P08247 X06389 CAA29686.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024