Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Western blot (1:5000 - 1:10000)
Specificity and Use
Full-length nitrated recombinant human a-synuclein.
Recognizes nitrated recombinant a-synuclein at Tyr39, Mr 14.5kD or nitrated b-Synuclein, Mr 17kD. Species cross-reactivity: Human.
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:5000-1:10000 detects a- or b-Synuclein recombinant protein nitrated at Tyr39. Nitrated and unnitrated a-synuclein recombinant protein were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-nitro-a/b-Synuclein (Tyr39) (1:5000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. nitrated a-Synuclein (~14.5kD). Note. The detected band at ~30kD represents the formation of o, o' -dityrosine cross-links occurring during nitration with. peroxynitrite. Immunohisto/cytochemistry: Detects nitrated a- and b-synuclein in human tissues.
0.05% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C