Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
Recombinant full-length oxidized human a-Synuclein, which shares 95% amino acid sequence homology with mouse and rat a-Synucleins.
Reacts with the C-terminal region1 of the nitrated/oxidized form of human a-Synuclein, as well as with mutated b-Synuclein.1 The binding of nSyn 12 is dependent upon nitration of either Tyr125 or Tyr136, with the recognition of Tyr136 dominating that of Tyr125.1 On Western blots, it identifies the target band at ~18kD. Reactivity has been confirmed with WT ONOO-, a recombinant wild-type form of a-Synuclein nitrated at Tyr39, 125, 133, and 136 by peroxynitrate, and with human brain extracts by Western blotting. Reactivity has also been confirmed with human DLB (dementia with Lewy bodies) and MSA (multiple system atrophy) brains by immunohistochemistry.1 Cross-reactivity with b-synuclein has been observed using bacterially-expressed and purified recombinant human proteins.1.
Suitable for use in ELISA, Western Blot, Immunohistochemistry. ELISA: 0.1-1.0 ug/ml. Western Blot: 1-3 ug/ml. Immunohistochemistry1:1-5 ug.