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SYNJ1 / Synaptojanin Antibody (aa147‑196) LS‑C101850
Synaptojanin antibody LS-C101850 is an unconjugated rabbit polyclonal antibody to Synaptojanin (SYNJ1) from human, bat, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C101850-50
50 µg (1 mg/ml)
Unavailable

Popular SYNJ1 / Synaptojanin Products

Antibody
Species: Rat
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Western blot Image
Species: Rat
Applications: IHC, Western blot
Anti-SYNJ1 Antibody at 1:2000 dilution + K562 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 173 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
All lanes: Anti-SYNJ1 Antibody (C-term) at 1:1000-1:2000 dilution. Lane 1: human brain lysate. Lane 2: HeLa whole cell lysate. Lane 3: mouse brain lysate. Lane 4: rat brain lysate. Lane 5: SW480 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 173 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

Synaptojanin antibody LS-C101850 is an unconjugated rabbit polyclonal antibody to Synaptojanin (SYNJ1) from human, bat, bovine and other species. Validated for WB.
About SYNJ1 / Synaptojanin
SYNJ1 / Synaptojanin is a phosphoinositide phosphatase that regulates levels of membrane phosphatidylinositol-4,5-bisphosphate. As such, expression of this enzyme may affect synaptic transmission and membrane trafficking. Multiple transcript variants encoding different isoforms have been found for this gene. NM_003895

SYNJ1 Antibody, INPP5G Antibody, KIAA0910 Antibody, Synaptojanin Antibody, Synaptojanin 1 Antibody, Synaptojanin-1 Antibody


Specifications

Target
Human SYNJ1 / Synaptojanin
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Dog, Guinea pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa147-196 of human SYNJ1 (O43426, NP_982271). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey, Galago, Marmoset, Bovine, Bat, Rabbit, Horse, Pig, Armadillo (100%); Mouse, Rat, Elephant, Dog, Guinea pig, Platypus (92%); Turkey, Zebra finch, Chicken (85%).
Epitope
aa147-196
Specificity
Human SYNJ1 / Synaptojanin
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

SYNJ1 / Synaptojanin antibody LS-C101850 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SYNJ1 / Synaptojanin antibody LS-C101850 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SYNJ1 / Synaptojanin antibody LS-C101850 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SYNJ1 / Synaptojanin antibody LS-C101850 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SYNJ1 / Synaptojanin antibody LS-C101850 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SYNJ1 / Synaptojanin antibody LS-C101850 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SYNJ1 / Synaptojanin antibody LS-C101850 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SYNJ1 / Synaptojanin antibody LS-C101850 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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