Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone FYN-59] (IgG) to Human SYN / FYN
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Human SYN / FYN
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Monoclonal [FYN-59]
IHC - Paraffin (15 µg/ml)
Specificity and Use
SYN / FYN antibody was raised against recombinant human FYN.
Partial recombinant human Fyn (aa 7-176) expressed in E coli
Immunohistochemistry: LS-B1657 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1657 was determined to be 15 ug/ml.
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml.
Unexpected role for the B cell-specific Src family kinase B lymphoid kinase in the development of IL-17-producing T cells. Laird RM, Laky K, Hayes SM. Journal of immunology (Baltimore, Md. : 1950). 2010 185:6518-27.
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions.
Anti-FYN antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1657 concentration 15 ug/ml.
Extracts from human PBMC were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against Fyn. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.