Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Western blot, Immunoprecipitation, ELISA, Functional Assay
Human SYN / FYN
Human, Mouse (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1 - 3 µg/ml)
ELISA (0.1 - 1 µg/ml)
Specificity and Use
SYN / FYN antibody was raised against a fusion protein consisting of aa1-206 of murine p59 fyn.
Recognizes a unique region of p59 fyn (Fyn) between aa26-75. Reactivity has been confirmed by immunoprecipitation or western blot analysis of extracts derived from human T cell hybridomas, Jurkat cells, mouse T cell hybridomas and recombinant Fyn.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. ELISA: 0.1-1 ug/ml. Immunoprecipitation, IP-Kinase Assay: 2-5 ug. Western Blot: 1-3 ug/ml.
PBS, pH 7.2, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions.