(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot (0.5 - 1 µg/ml)
SYK antibody was raised against synthetic peptide corresponding to the cytoplasmic domain of human Syk protein (aa 313-339). Cellular Localization: Cell membrane MW of antigen: 72kD.
Recognizes human Syk. Does not react with porcine or mouse.
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 2 ug/mg protein lysate. Use Protein A. Immunohistochemistry (formalin/paraffin): 1:50 for 30 minutes at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Western Blot: 0.5-1.0 ug/ml for 2hrs at RT. Positive control: Raji, K562, HL-60, U937 cells or lymphoma.
PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.