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Anti-SYCP3 / SCP3 Antibody (C-Terminus) IHC-plus™ LS-B175

Note: This antibody replaces LS-C2887, LS-C91258

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SYCP3 / SCP3 Antibodies

Anti-SYCP3 / SCP3 Antibody (C-Terminus) IHC-plus™ LS-B175
Rabbit Polyclonal to Human SYCP3 / SCP3
Human, Mouse, Rat, Bovine, Pig
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SYCP3 / SCP3 Antibody (N-Terminus) IHC-plus™ LS-B6637
Rabbit Polyclonal (IgG) to Human SYCP3 / SCP3
Human, Monkey
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SYCP3 / SCP3 Antibody (aa27-128, clone 6F9C5) LS-C285849
Mouse Monoclonal [clone 6F9C5] (IgG1) to Human SYCP3 / SCP3
Human
IHC - Paraffin, ICC, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SYCP3 / SCP3
Human, Mouse, Rat, Bovine, Pig
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SYCP3 / SCP3
Rabbit
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • IHC - Frozen (1:200 - 1:500)
  • Immunofluorescence
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

SYCP3 / SCP3 antibody was raised against a synthetic peptide made to the C-terminal region of the human SCP3 protein
C-Terminus
SCP3.
Immunohistochemistry: LS-B175 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B175 was determined to be 20 ug/ml.

Packaging

PBS, 0.09% sodium azide.
Store at 4°C. Do not freeze.
For research use only.

About SYCP3 / SCP3

Q8IZU3 NM_153694 NP_710161.1

SYCP3 Antibody, SCP3 Antibody, SPGF4 Antibody, Synaptonemal complex protein 3 Antibody, SCP-3 Antibody, COR1 Antibody

Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. Required for efficient phosphorylation of HORMAD1 and HORMAD2.

Immunohistochemistry

Immunohistochemistry
Anti-SYCP3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B175 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Immunofluorescence
SCP3 labeled in mouse pachytene preparation (red), using LS-B175. CDK2 staining, near teleomeres, is also present (green). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western blot of SCP3 in mouse testis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/8/2016

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