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Catalog Number Size Price
LS-C60086-100 100 µg (1 mg/ml) $567 
SWI6 Antibody - Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
SWI6 Antibody - Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.
SWI6 Antibody - Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
SWI6 Antibody - Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.
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Polyclonal Rabbit anti‑S. pombe SWI6 Antibody (WB) LS‑C60086

Polyclonal Rabbit anti‑S. pombe SWI6 Antibody (WB) LS‑C60086

Antibody:
SWI6 Rabbit anti-S. pombe Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
S. pombe, Schizosaccharomyces pombe
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C60086-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SWI6 Rabbit anti-S. pombe Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
S. pombe, Schizosaccharomyces pombe
Format:
Unconjugated, Unmodified

Specifications

Description
SWI6 antibody LS-C60086 is an unconjugated rabbit polyclonal antibody to SWI6 from s. pombe. It is reactive with s. pombe and schizosaccharomyces pombe. Validated for ELISA and WB.
Target
S. pombe SWI6
Synonyms
SWI6 | SDS11 | Swi6p | PSL8
Host
Rabbit
Reactivity
S. pombe, Schizosaccharomyces pombe (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This anti-Swi6 Antibody was prepared from whole rabbit antiserum produced by repeated immunizations with a synthetic peptide corresponding aa 314-328 of S.pombe Swi6 protein.
Specificity
BLAST analysis indicates that cross reactivity with homologues of this protein from other sources is not likely.
Applications
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:30000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kD in size corresponding to Swi6 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SWI6
P09959 NP_013283.1

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Requested From: United States
Date Requested: 4/18/2024