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SWI6 Antibody (aa314‑328) LS‑C60086 Best Seller
SWI6 antibody LS-C60086 is an unconjugated rabbit polyclonal antibody to s. pombe SWI6. Validated for ELISA and WB.
Catalog
Size
Price
LS-C60086-100
100 µg
$425
Description
SWI6 antibody LS-C60086 is an unconjugated rabbit polyclonal antibody to s. pombe SWI6. Validated for ELISA and WB.
Target
S. pombe SWI6
Host
Rabbit
Reactivity
S. pombe (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:30000)
Immunogen
SWI6 antibody was raised against synthetic peptide corresponding aa 314-328 of S. pombe Swi6 protein.
Epitope
aa314-328
Specificity
BLAST analysis indicates that cross reactivity with homologs of this protein from other sources is not likely
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kD in size corresponding to Swi6 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
About SWI6
P09959 NP_013283.1

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Images

Western blot

Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.
Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.

Western blot

Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.
Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.

Western blot

Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.
Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.

Western blot

Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.
Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.

Requested From: United States
Date Requested: 12/12/2018
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