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Anti-SWI6 Antibody (aa314-328) LS-C60086


Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular SWI6 Antibodies

Anti-SWI6 Antibody (aa314-328) LS-C60086
Rabbit Polyclonal to S. pombe SWI6
S. pombe
Western blot, ELISA
Western blot Image

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Rabbit Polyclonal to S. pombe SWI6
S. pombe
Western blot, ELISA


S. pombe SWI6
S. pombe (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:30000)

Specificity and Use

SWI6 antibody was raised against synthetic peptide corresponding aa 314-328 of S. pombe Swi6 protein.
BLAST analysis indicates that cross reactivity with homologs of this protein from other sources is not likely
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kD in size corresponding to Swi6 protein by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About SWI6

P09959 NP_013283.1

SWI6 Antibody, SDS11 Antibody, Swi6p Antibody, PSL8 Antibody

Transcription cofactor, forms complexes with DNA-binding proteins Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulated by phosphorylation; potential Cdc28p substrate.

Western blot

Western blot
Anti-Swi6 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kD corresponding to S. pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Western blot
Schematic model of S. pombe centromere cluster in interphase cells. The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.

Requested From: United States
Date Requested: 10/23/2016

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