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Catalog Number Size Price
LS-C18914-500 500 µg (5 mg/ml) $606 
SUMO3 Antibody - Anti-Human SUMO-3 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human SUMO-3 antibody to detect GFP-SUMO fusion proteins (arrowheads). Panel A. Increasing concentrations of human GFP-SUMO-3 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1:1000 dilution of anti-hSUMO-3 antibody in 5% non-fat dry milk in TTBS. Detection occurred using a 1:1000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Panel B. Specificity of the antibody was confirmed by SDS-PAGE of 5 ug of various GFP-SUMO constructs followed by transfer onto nitrocellulose. Lanes: 1. MW marker, 2. GFP-human SUMO-3, 3. GFP-human SUMO-1, 4. GFP-yeast SUMO, 5. GFP-Arabidopsis thaliana, SUMO-1, 6. GFP- Arabidopsis thaliana SUMO-2, 7. GFP-tomato SUMO. After blocking for 1 hour with 5% non-fat dry milk in TTBS, the blot was probed overnight at 4°C with anti-hSUMO-3 antibody diluted and detected as above. Only the human GFP-SUMO-3 band was visualized by chemiluminescence, and no cross-reactivity with other SUMO family members was observed.

Polyclonal Rabbit anti‑Human SUMO3 Antibody (IF, WB) LS‑C18914

Polyclonal Rabbit anti‑Human SUMO3 Antibody (IF, WB) LS‑C18914

Antibody:
SUMO3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$606
LS-C18914-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SUMO3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SUMO3 antibody LS-C18914 is an unconjugated rabbit polyclonal antibody to human SUMO3. Validated for ELISA, IF and WB.
Target
Human SUMO3
Synonyms
SUMO3 | SMT3A | SMT3 homolog 1 | Smt3B | SMT3H1 | SUMO-2 | SUMO-3 | Ubiquitin-like protein SMT3A | Ubiquitin-like protein SMT3B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO-3 protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:4000 - 1:20000)
Usage
This purified polyclonal antibody reacts with human SUMO-3 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11.6 kD in size corresponding to human SUMO-3 by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. Restore with 0.1 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SUMO3
P55854 NM_006936 NP_008867.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024