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Anti-SUMO3 Antibody LS-C18914


Wt. Vol. Conc. Price
500 µg 500 µl - Unavailable

Most Popular SUMO3 Antibodies

Anti-SUMO3 Antibody LS-C18914
Rabbit Polyclonal to Human SUMO3
Western blot, ELISA
Western blot Image
Anti-SUMO3 Antibody (clone 76AT630.91.31) LS-C96462
Mouse Monoclonal [clone 76AT630.91.31] (IgG1) to Human SUMO3
Western blot
Western blot Image
Anti-SUMO3 Antibody (C-Terminus) LS-C102761
Rabbit Polyclonal (IgG) to Human SUMO3
Western blot
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SUMO3
Western blot, ELISA


Human SUMO3
Human (tested or 100% immunogen sequence identity)
Purified IgG


  • Western blot (1:500 - 1:2000)
  • ELISA (1:4000 - 1:20000)

Specificity and Use

SUMO3 antibody was raised against recombinant human SUMO3.
Recombinant human SUMO-3 protein.
This purified polyclonal antibody reacts with human SUMO-3 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11.6 kD in size corresponding to human SUMO-3 by western blotting in the appropriate cell lysate or extract.


500 µl After adding water, will consist of 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide
Store vial at 4 C prior to restoration. Restore with 0.1 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About SUMO3

P55854 NM_006936 NP_008867.2

SUMO3 Antibody, SMT3A Antibody, SMT3 homolog 1 Antibody, Smt3B Antibody, SMT3H1 Antibody, SUMO-2 Antibody, SUMO-3 Antibody, Ubiquitin-like protein SMT3A Antibody, Ubiquitin-like protein SMT3B Antibody

Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction.

Western blot

Western blot
Anti-Human SUMO-3 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human SUMO-3 antibody to detect GFP-SUMO fusion proteins (arrowheads). Panel A. Increasing concentrations of human GFP-SUMO-3 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1:1000 dilution of anti-hSUMO-3 antibody in 5% non-fat dry milk in TTBS. Detection occurred using a 1:1000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Panel B. Specificity of the antibody was confirmed by SDS-PAGE of 5 ug of various GFP-SUMO constructs followed by transfer onto nitrocellulose. Lanes: 1. MW marker, 2. GFP-human SUMO-3, 3. GFP-human SUMO-1, 4. GFP-yeast SUMO, 5. GFP-Arabidopsis thaliana, SUMO-1, 6. GFP- Arabidopsis thaliana SUMO-2, 7. GFP-tomato SUMO. After blocking for 1 hour with 5% non-fat dry milk in TTBS, the blot was probed overnight at 4°C with anti-hSUMO-3 antibody diluted and detected as above. Only the human GFP-SUMO-3 band was visualized by chemiluminescence, and no cross-reactivity with other SUMO family members was observed.

Requested From: 
Date Requested: 3/28/2017

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