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Anti-SUMO1 / SMT3 Antibody (N-Terminus) IHC-plus™ LS-B594

Note: This antibody replaces LS-C19650, LS-C56453, LS-C42498, LS-C132038

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SUMO1 / SMT3 Antibodies

Anti-SUMO1 / SMT3 Antibody (N-Terminus) IHC-plus™ LS-B594
Rabbit Polyclonal to Human SUMO1 / SMT3
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SUMO1 / SMT3 Antibody (clone 5B12) LS-C179834
Mouse Monoclonal [clone 5B12] (IgG1) to Human SUMO1 / SMT3
Human, Mouse, Rat
ICC, Western blot
Unconjugated
Western blot Image
Anti-SUMO1 / SMT3 Antibody (Biotin) LS-C282588
Mouse Monoclonal (IgG1) to Human SUMO1 / SMT3
Human
Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SUMO1 / SMT3
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human SUMO1 / SMT3
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot

Specificity and Use

SUMO1 / SMT3 antibody was raised against synthetic peptide from human SUMO1.
LS-E30284 - Liquid - 50 µg - $145.00
N-Terminus
14 amino acid peptide from near the amino terminus of human sumo
Immunohistochemistry: LS-B594 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B594 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About SUMO1 / SMT3

P63165 NM_003352 NP_003343.1

SUMO1 Antibody, GAP modifying protein 1 Antibody, GMP1 Antibody, OFC10 Antibody, Sentrin Antibody, SMT3 homolog 3 Antibody, SMT3C Antibody, SMT3H3 Antibody, SUMO-1 Antibody, Ubiquitin-like protein UBL1 Antibody, PIC1 Antibody, Ubiquitin-like 1 (sentrin) Antibody, UBL1 Antibody, DAP1 Antibody, GAP-modifying protein 1 Antibody, Ubiquitin-like protein SMT3C Antibody

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4.

Immunohistochemistry

Immunohistochemistry
Anti-SUMO1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B594 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-SUMO1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B594 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of Sumo in HL60 cells with Sumo antibody at 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of Sumo in HL60 cells with Sumo antibody at 20 ug/ml.

Western blot

Western blot
Western blot of sumo in HL-60 cell lysate with sumo antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
 

Requested From: United States
Date Requested: 12/9/2016

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