Human SUMO1 / SMT3

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Unmodified

This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO protein.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

• Western blot (1:500 - 1:3000)
• ELISA (1:5000 - 1:25000)

This purified polyclonal antibody reacts with human SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. An 11.6 kD band corresponding to human SUMO is detected. Most human cell lysates can be used as a positive control without induction or stimulation.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P63165 NCBI: NM_003352 NP_003343.1