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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SUCLG1 / GALPHA Antibody (aa1‑346) LS‑C186052
GALPHA antibody LS-C186052 is an unconjugated rabbit polyclonal antibody to human GALPHA (SUCLG1). Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C186052-100
100 µl (1 mg/ml)
Unavailable

Popular SUCLG1 / GALPHA Products

Antibody
Species: Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation
SUCLG1 / GALPHA antibody Western Blot of U937.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Horse, Rabbit
Applications: Western blot
SUCLG1 / GALPHA antibody Western Blot of Human Lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Guinea pig, Horse, Pig, Rabbit
Applications: Western blot
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, ELISA

Product Description

GALPHA antibody LS-C186052 is an unconjugated rabbit polyclonal antibody to human GALPHA (SUCLG1). Validated for ICC, IHC and WB.
About SUCLG1 / GALPHA
Catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. The nature of the beta subunit determines the nucleotide specificity. P53597 NM_003849 NP_003840.2

SUCLG1 Antibody, SCS-alpha Antibody, SUCLA1 Antibody, GALPHA Antibody, MTDPS9 Antibody


Specifications

Target
Human SUCLG1 / GALPHA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:1000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SUCLG1 / GALPHA antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 346 of SUCLG1 (SwissProt P53597).
Epitope
aa1-346
Specificity
Human SUCLG1 / GALPHA
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Customer Reviews (0)


Images


Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using SUCLG1 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using SUCLG1 antibody at 1:100 dilution.

Immunocytochemistry

SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. SUCLG1 protein stained by SUCLG1 antibody diluted at 1:500.
SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. SUCLG1 protein stained by SUCLG1 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: A431. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: A431. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:1000

Western blot

SUCLG1 antibody detects SUCLG1 protein by Western blot analysis. A.50 ug rat brain lysate/extract. 10 % SDS-PAGE. SUCLG1 antibody dilution:1:3000
SUCLG1 antibody detects SUCLG1 protein by Western blot analysis. A.50 ug rat brain lysate/extract. 10 % SDS-PAGE. SUCLG1 antibody dilution:1:3000

Western blot

Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:10000.
Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:10000.

Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using SUCLG1 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using SUCLG1 antibody at 1:100 dilution.

Immunocytochemistry

SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. SUCLG1 protein stained by SUCLG1 antibody diluted at 1:500.
SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. SUCLG1 protein stained by SUCLG1 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: A431. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: A431. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:1000

Western blot

SUCLG1 antibody detects SUCLG1 protein by Western blot analysis. A.50 ug rat brain lysate/extract. 10 % SDS-PAGE. SUCLG1 antibody dilution:1:3000
SUCLG1 antibody detects SUCLG1 protein by Western blot analysis. A.50 ug rat brain lysate/extract. 10 % SDS-PAGE. SUCLG1 antibody dilution:1:3000

Western blot

Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:10000.
Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:10000.

Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using SUCLG1 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using SUCLG1 antibody at 1:100 dilution.

Immunocytochemistry

SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. SUCLG1 protein stained by SUCLG1 antibody diluted at 1:500.
SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. SUCLG1 protein stained by SUCLG1 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: A431. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: A431. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:1000

Western blot

SUCLG1 antibody detects SUCLG1 protein by Western blot analysis. A.50 ug rat brain lysate/extract. 10 % SDS-PAGE. SUCLG1 antibody dilution:1:3000
SUCLG1 antibody detects SUCLG1 protein by Western blot analysis. A.50 ug rat brain lysate/extract. 10 % SDS-PAGE. SUCLG1 antibody dilution:1:3000

Western blot

Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:10000.
Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:10000.

Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using SUCLG1 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using SUCLG1 antibody at 1:100 dilution.

Immunocytochemistry

SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. SUCLG1 protein stained by SUCLG1 antibody diluted at 1:500.
SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. SUCLG1 protein stained by SUCLG1 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: A431. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: A431. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:1000

Western blot

SUCLG1 antibody detects SUCLG1 protein by Western blot analysis. A.50 ug rat brain lysate/extract. 10 % SDS-PAGE. SUCLG1 antibody dilution:1:3000
SUCLG1 antibody detects SUCLG1 protein by Western blot analysis. A.50 ug rat brain lysate/extract. 10 % SDS-PAGE. SUCLG1 antibody dilution:1:3000

Western blot

Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:10000.
Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. SUCLG1 / GALPHA antibody diluted at 1:10000.


Requested From: 
Date Requested: 6/20/2018

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