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Anti-STUB1 / CHIP Antibody (N-Terminus) LS-C110748

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Most Popular STUB1 / CHIP Antibodies

Anti-STUB1 / CHIP Antibody (aa219-231) IHC-plus™ LS-B6443
Goat Polyclonal to Human STUB1 / CHIP
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Chicken, Xenopus
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-STUB1 / CHIP Antibody (aa243-271, Biotin) LS-C241389
Rabbit Polyclonal (IgG) to Human STUB1 / CHIP
Human
Western blot, Flow Cytometry, ELISA
Biotin Conjugated
Anti-STUB1 / CHIP Antibody (aa1-50) LS-C288366
Rabbit Polyclonal (IgG) to Human STUB1 / CHIP
Human, Mouse, Rat, Bovine, Guinea pig, Pig
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human STUB1 / CHIP
Human, Monkey, Mouse, Rat, Bat, Guinea pig
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human STUB1 / CHIP
Rabbit
Human, Monkey, Mouse, Rat, Bat, Guinea pig (tested or 100% immunogen sequence identity)
Bovine, Pig, Zebrafish (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

STUB1 / CHIP antibody was raised against synthetic peptide from N-Terminus of human STUB1 (Q9UNE7, NP_005852). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Bat, Opossum, Guinea pig (100%); Bovine, Pig, Zebrafish (92%); Chicken, Stickleback (85%); Xenopus (82%).
LS-E15847 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110748. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
N-Terminus
Human STUB1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About STUB1 / CHIP

Q9UNE7 NM_005861 NP_005852.2

STUB1 Antibody, Antigen NY-CO-7 Antibody, CHIP Antibody, HSPABP2 Antibody, NY-CO-7 Antibody, SDCCAG7 Antibody, UBOX1 Antibody, CLL-associated antigen KW-8 Antibody

E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90.

Western blot

Western blot
STUB1 antibody LS-C110748 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/28/2017

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