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Anti-STUB1 / CHIP Antibody (aa236-263) LS-C161582

Catalog	Size	Price
LS-C161582-400	400 µl	Unavailable

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Anti-STUB1 / CHIP Antibody (aa253-303) IHC-plus™ LS-B11567

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Antibody:	Rabbit Polyclonal to Human STUB1 / CHIP
Reactivity:	Human
Application:	Western blot, Flow Cytometry
Format:	Unconjugated

Details

Target:	Human STUB1 / CHIP
Host:	Rabbit
Reactivity:	Human (tested or 100% immunogen sequence identity)
Predicted:	Mouse, Chicken (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated
Purification:	Protein A purified
Modifications:	Unmodified

Applications

Approved:

Western blot (1:1000)
Flow Cytometry (1:10 - 1:50)

Specificity and Use

Blocking Peptide:

LS-E1469 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C161582. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa236-263

Specificity:

This STUB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-263 amino acids from the C-terminal region of human STUB1.

Packaging

Presentation:	PBS, 0.09% sodium azide
Storage:	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions:	For research use only.

About STUB1 / CHIP

Uniprot: Q9UNE7 NCBI: NM_005861 NP_005852.2

Synonyms: STUB1 Antibody, Antigen NY-CO-7 Antibody, CHIP Antibody, HSPABP2 Antibody, NY-CO-7 Antibody, SDCCAG7 Antibody, UBOX1 Antibody, CLL-associated antigen KW-8 Antibody

Description: E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90.

(More About STUB1 / CHIP)

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Western blot

STUB1 Antibody western blot of 293 cell line lysates (35 ug/lane). The STUB1 antibody detected the STUB1 protein (arrow).

Flow Cytometry

STUB1 Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From:
Date Requested: 8/18/2017

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