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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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STUB1 / CHIP Antibody (aa219‑231) IHC‑plus™ LS‑B6443 Best Seller
Note: This antibody replaces LS-C55538
CHIP antibody LS-B6443 is an unconjugated goat polyclonal antibody to CHIP (STUB1) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6443-50
50 µg (0.5 mg/ml)
Unavailable

Popular STUB1 / CHIP Products

Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Antibody
Species: Human, Mouse, Rat, Bovine, Guinea pig, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Anti-STUB1 / CHIP antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10877 concentration 7.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA892480LA01HU at dilution of 1:100
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

CHIP antibody LS-B6443 is an unconjugated goat polyclonal antibody to CHIP (STUB1) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About STUB1 / CHIP
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Q9UNE7 NM_005861 NP_005852.2

STUB1 Antibody, Antigen NY-CO-7 Antibody, CHIP Antibody, HSPABP2 Antibody, NY-CO-7 Antibody, SDCCAG7 Antibody, UBOX1 Antibody, CLL-associated antigen KW-8 Antibody


Specifications

Target
Human STUB1 / CHIP
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 - 3.75 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
STUB1 / CHIP antibody was raised against synthetic peptide C-DAFISENGWVEDY from the C-terminus of human STUB1 (NP_005852.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Xenopus (100%); Zebrafish (92%).
Blocking Peptide
LS-E25309 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6443. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa219-231
Specificity
Human STUB1.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-STUB1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6443 concentration 2.5 ug/ml.
Anti-STUB1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6443 concentration 2.5 ug/ml.

Western blot

Staining (0.3?g/ml) of Human Muscle lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (0.3?g/ml) of Human Muscle lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (0.3 ug/ml) of Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-STUB1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6443 concentration 2.5 ug/ml.
Anti-STUB1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6443 concentration 2.5 ug/ml.

Western blot

Staining (0.3?g/ml) of Human Muscle lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (0.3?g/ml) of Human Muscle lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (0.3 ug/ml) of Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-STUB1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6443 concentration 2.5 ug/ml.
Anti-STUB1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6443 concentration 2.5 ug/ml.

Western blot

Staining (0.3?g/ml) of Human Muscle lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (0.3?g/ml) of Human Muscle lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (0.3 ug/ml) of Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-STUB1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6443 concentration 2.5 ug/ml.
Anti-STUB1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6443 concentration 2.5 ug/ml.

Western blot

Staining (0.3?g/ml) of Human Muscle lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (0.3?g/ml) of Human Muscle lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (0.3 ug/ml) of Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.


Requested From: 
Date Requested: 6/19/2018

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