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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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STK4 Antibody (aa276‑487) LS‑C16613
STK4 antibody LS-C16613 is an unconjugated rabbit polyclonal antibody to STK4 from human, mouse and rat. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C16613-100
100 µg
Unavailable

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Product Description

STK4 antibody LS-C16613 is an unconjugated rabbit polyclonal antibody to STK4 from human, mouse and rat. Validated for IP and WB.
About STK4
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Q13043 NM_006282 NP_006273.1

STK4 Antibody, Mammalian sterile 20-like 1 Antibody, MST-1 Antibody, MST2 Antibody, KRS2 Antibody, Serine/threonine kinase 4 Antibody, TIIAC Antibody, STE20-like kinase MST1 Antibody, YSK3 Antibody, Kinase responsive to stress 2 Antibody


Specifications

Target
Human STK4
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
STK4 antibody was raised against gST fusion protein corresponding to residues 276-487 of human Mst1/Krs2.
Epitope
aa276-487
Specificity
Recognizes Mst1/Krs2, Mr 56kD. An additional protein may be detected in some cell lines, Mr 64kD. Species cross-reactivity: Human, mouse and rat.
Usage
Immunoprecipitation Kinase: This antibody has been reported to immunoprecipitate active MST1/Krs2 from Cos cells transiently transfected with MST1/Krs2.1,2. Western Blot Analysis: 0.5-2 ug/ml detects MST1/Krs2 in RIPA lysates from A431. A previous lot detected MST1/Krs2 in RIPA lysates from HeLa cells. Western Blot Analysis: Representative blot from a previous lot. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MST1/Krs2 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. MST1/Krs2 ~56kD).
Presentation
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 7/19/2018

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