STK4 antibody LS-C16613 is an unconjugated rabbit polyclonal antibody to STK4 from human, mouse and rat. Validated for IP and WB.
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
STK4 antibody was raised against gST fusion protein corresponding to residues 276-487 of human Mst1/Krs2.
Recognizes Mst1/Krs2, Mr 56kD. An additional protein may be detected in some cell lines, Mr 64kD. Species cross-reactivity: Human, mouse and rat.
Immunoprecipitation Kinase: This antibody has been reported to immunoprecipitate active MST1/Krs2 from Cos cells transiently transfected with MST1/Krs2.1,2. Western Blot Analysis: 0.5-2 ug/ml detects MST1/Krs2 in RIPA lysates from A431. A previous lot detected MST1/Krs2 in RIPA lysates from HeLa cells. Western Blot Analysis: Representative blot from a previous lot. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MST1/Krs2 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. MST1/Krs2 ~56kD).
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.