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Anti-STK39 / SPAK Antibody (aa346-376) LS-C98726

Catalog Size Price
LS-C98726-400 400 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human STK39 / SPAK
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human STK39 / SPAK
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)

Specificity and Use

LS-E9398 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C98726. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa346-376
This SPAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-376 amino acids from the Central region of human SPAK.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About STK39 / SPAK

Q9UEW8 NM_013233 NP_037365.2

STK39 Antibody, Serine threonine kinase 39 Antibody, STE20/SPS1 homolog Antibody, Ste20-like protein kinase Antibody, DCHT Antibody, SPAK Antibody, Ste-20-related kinase Antibody

STK39 / SPAK is a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot
SPAK Antibody (A363) western blot of U937 cell line lysates (35 ug/lane). The SPAK antibody detected the SPAK protein (arrow).

Western blot

Western blot
Western blot of SPAK (arrow) using rabbit polyclonal SPAK Antibody (A363). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPAK gene.

Western blot

Western blot
Western blot of SPAK (arrow) using rabbit polyclonal SPAK Antibody (A363). 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPAK gene.

Western blot

Western blot
Western blot of lysate from HepG2 cell line, using SPAK Antibody (A363). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot
Western blot of lysate from 293 cell line, using SPAK Antibody (A363). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot

Western blot
Western blot of anti-SPAK antibody in mouse liver tissue lysate. SPAK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Requested From: 
Date Requested: 10/18/2017

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