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Anti-STI1 / STIP1 Antibody (clone 1E3) IHC-plus™ LS-B6208

Note: This antibody replaces LS-C133667

Ordering

Wt. Vol. Conc. Price
50 µg - 0.782 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular STI1 / STIP1 Antibodies

Anti-STI1 / STIP1 Antibody (clone 4B6) LS-C133666
Mouse Monoclonal [clone 4B6] (IgG2a,k) to Human STI1 / STIP1
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunofluorescence Image
Anti-STI1 / STIP1 Antibody (clone 1E3) IHC-plus™ LS-B6208
Mouse Monoclonal [clone 1E3] (IgG2a,k) to Human STI1 / STIP1
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-STI1 / STIP1 Antibody (clone 2E1) IHC-plus™ LS-B6209
Mouse Monoclonal [clone 2E1] (IgG1,k) to Human STI1 / STIP1
Human
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1E3] (IgG2a,k) to Human STI1 / STIP1
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human STI1 / STIP1
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1E3]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (3 - 5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

STI1 / STIP1 antibody was raised against sTIP1 (NP_006810, 445 a.a. ~ 544 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human STIP1
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About STI1 / STIP1

P31948 NM_006819 NP_006810.1

STIP1 Antibody, HOP Antibody, IEF-SSP-3521 Antibody, NY-REN-11 antigen Antibody, p60 Antibody, STI1 Antibody, Hsc70/Hsp90-organizing protein Antibody, Hsp70/Hsp90-organizing protein Antibody, STI1L Antibody

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).

Immunohistochemistry

Immunohistochemistry
Anti-STIP1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6208 concentration 3 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-STIP1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6208 concentration 3 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
Antibody LS-B6208, Western blot of STIP1 expression in PC-12.

Western blot

Western blot
Antibody LS-B6208, Western blot of STIP1 expression in NIH/3T3.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged STIP1 is approximately 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/7/2016

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