Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Human, Rat, Bovine, Dog, Hamster, Horse, Pig, Chicken
(tested or 100% immunogen sequence identity)
Bat (at least 90% immunogen sequence identity)
Western blot (1:1000 - 1:2000)
STAT5B antibody was raised against synthetic peptide (MDSQWIPHAQS) corresponding to amino acids 776-786 of mouse STAT5B (KLH). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig, Chicken (100%); Panda, Bat, Opossum (91%).
Recognizes mouse STAT5B at ~94kD. Species cross-reactivity: Rat, human and chicken.
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
STAT5B is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones.