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Anti-STAT5A Antibody (phospho-Tyr694) LS-C96983

Catalog Size Price
LS-C96983-400 400 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human STAT5A
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human STAT5A
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)

Specificity and Use

LS-E7325 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C96983. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
pTyr694
This STAT5a Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y694 of human STAT5a.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About STAT5A

P42229 NM_003152 NP_003143.2

STAT5A Antibody, MGF Antibody, STAT5 Antibody

STAT5A is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of an SY5Y cell stained Phospho-STAT5a antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated Phospho-STAT5a primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min)(not shown). Phospho-STAT5a immunoreactivity is localized exclusively to the nucleus.

Western blot

Western blot
Western blot of anti-Phospho-STAT5 Pabin HepG2 cell line lysate (35 ug/lane). Phospho-STAT5(arrow) was detected using the purified antibody.

Requested From: 
Date Requested: 8/21/2017

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