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Anti-STAT5A Antibody (phospho-Tyr694, clone 5F6.F1) LS-C154006

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular STAT5A Antibodies

Anti-STAT5A Antibody (aa774-794, PE) LS-C76024
Mouse Monoclonal (IgG1) to Human STAT5A
Human
Flow Cytometry
PE Conjugated
Anti-STAT5A Antibody IHC-plus™ LS-B8739
Rabbit Polyclonal (IgG) to Human STAT5A
Human, Mouse, Rat, Bovine, Pig
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5F6.F1] (IgG) to Mouse STAT5A
Mouse, Human, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Mouse STAT5A
Mouse
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
IgG Monoclonal [5F6.F1]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (20 - 40 µg/ml)
  • Immunofluorescence (1:50 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA

Specificity and Use

STAT5A antibody was raised against anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
pTyr694
This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.
Phospho STAT5 pY694 monoclonal antibody is suitable for Immunofluorescence microscopy, ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About STAT5A

P42229 NM_003152 NP_003143.2

STAT5A Antibody, MGF Antibody, STAT5 Antibody

STAT5A is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones.

Immunohistochemistry

Immunohistochemistry
Anti-Stat5 pY694 Monoclonal Antibody - Immunohistochemistry. Immunohistochemistry using Anti-Stat5 pY694 monoclonal antibody shows detection of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used a dilution to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.

Immunofluorescence

Immunofluorescence
Anti-Stat5 pY694 Monoclonal Antibody - Immunofluorescent Microscopy. Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody (1:500) detecting Stat5 in 3T3 cells (immunofluorescent STED microscopy). Red represents Stat5 pY694 protein. Green represents tubulin.

Western blot

Western blot
Anti-Stat5 pY694 Monoclonal Antibody - Western Blot. Western blot of Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kD) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4C. For detection DyLight800 conjugated Gt-a-Mouse IgG was used at a 1:20000 dilution for 30 min at room temperature followed by visualization using a VersaDoc MP 4000 imaging system (Bio-Rad).
 

Requested From: United States
Date Requested: 12/8/2016

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